Zimmerman AE, Howard-Varona C, Needham DM, John SG, Worden AZ, Sullivan MB, et al. Metabolic and biogeochemical consequences of viral infection in aquatic ecosystems. Nat Rev Microbiol. 2020;18(1):21–34. https://doi.org/10.1038/s41579-019-0270-x. Frozen Pokémon cannot take any action and are immune to all damage except Fire-type and Blast Seed. Pokémon will thaw out after a few turns. Physical properties The three-dimensional crystal structure of H 2O ice I h (c) is composed of bases of H 2O ice molecules (b) located on lattice points within the two-dimensional hexagonal space lattice (a). [8] [9]

Knowlton C, Veerapaneni R, D’Elia T, Rogers SO. Microbial analyses of ancient ice core sections from Greenland and Antarctica. Biology (Basel). 2013;2:206–32. In I Choose You!, Corey's Gengar was frozen after Neesha had her Blastoise use Ice Beam on it, forcing Corey to recall it and send his Venusaur out in its place. Capella-Gutierrez S, Silla-Martinez JM, Gabaldon T. trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics. 2009;25(15):1972–3. https://doi.org/10.1093/bioinformatics/btp348. In Lost at the League!, during the Vertress Conference, Virgil's Glaceon simultaneously froze and defeated a Trainer's Reuniclus with Blizzard.In The Road to the Semifinals!, during Lana's second-round battle in the Manalo Conference, Lana's Primarina briefly froze the forearms of Guzma's Golisopod with Icy Wind before the latter simply broke the ice around its arms with its sharp claws. Later on in the round, Golisopod's legs were frozen to the ground by another Icy Wind. Santibanez PA, Maselli OJ, Greenwood MC, Grieman MM, Saltzman ES, McConnell JR, et al. Prokaryotes in the WAIS Divide ice core reflect source and transport changes between Last Glacial Maximum and the early Holocene. Glob Chang Biol. 2018;24(5):2182–97. https://doi.org/10.1111/gcb.14042. In Arrival of a Rival!, during the Jubilife City Pokémon Contest, Dawn's Buneary froze a Coordinator's Bidoof with an Ice Beam. A tetragonal crystalline phase. Formed by cooling water to 270K at 1.1GPa. Exhibits Debye relaxation. [41]

In Finals Not For the Faint-Hearted!, during the Lumiose Conference finals, Ash's Noivern got part of his wing frozen by an Ice Beam from Alain's Weavile, sending the Sound Wave Pokémon falling into a river and allowing Weavile to finish him off with a Night Slash. In Leaping Toward the Dream!, Goh's Scizor was frozen by an Alolan Ninetales's Ice Beam, forcing Goh to recall it. Abyzov SS, Mitskevich IN, Poglazova MN. Microflora of the deep glacier horizons of Central Antarctica. Microbiology. 1998;67:451–8. Kim J, Chhetri G, Kim I, Lee B, Jang W, Kim MK, et al. Methylobacterium terricola sp. nov., a gamma radiation-resistant bacterium isolated from gamma ray-irradiated soil. Int J Syst Evol Microbiol. 2020;70(4):2449–56. https://doi.org/10.1099/ijsem.0.004054. Skvortsov T, de Leeuwe C, Quinn JP, McGrath JW, Allen CC, McElarney Y, et al. Metagenomic characterisation of the viral community of Lough Neagh, the largest freshwater lake in Ireland. PLoS One. 2016;11(2):e0150361. https://doi.org/10.1371/journal.pone.0150361.Water covers more than 70 percent of Earth's surface. Slightly more than two percent of Earth's water is frozen into ice; almost all of this ice is in glaciers, which are huge masses of ice. Today, glaciers are found in many mountainous areas and in the polar regions of Earth. Hesse U, van Heusden P, Kirby BM, Olonade I, van Zyl LJ, Trindade M. Virome assembly and annotation: a surprise in the Namib desert. Front Microbiol. 2017;8:13. In Ash, Iris, and Trip: Then There Were Three!, during Ash's match against Iris in the Junior Cup semifinals, Ash's Krokorok was briefly frozen by an Ice Beam from Iris's Dragonite before the latter sent him flying with a Flamethrower. Several OTUs were unique in the removed samples, including one OTU belonging to the genus Acinetobacter for sample D13.3, as well as two OTUs within the genus Hymenobacter and one unclassified bacterial OTU for sample D13.5 (Additional file 2: Table S1). We posit that these OTUs (<1.0%) might be contaminants removed from the ice core surface. We also note that there may also be natural variations in microbial communities across the same cross section of an ice core (here they were represented by cut, wash, and inner samples from the same depth), as uneven horizontal distribution of dust, nutrients, and microbes in an ice core is not unexpected and may reflect variation in deposition. Microbial profiles potentially differ between the PS and S3 ice cores Ice possesses a regular crystalline structure based on the molecule of water, which consists of a single oxygen atom covalently bonded to two hydrogen atoms, or H–O–H. However, many of the physical properties of water and ice are controlled by the formation of hydrogen bonds between adjacent oxygen and hydrogen atoms; while it is a weak bond, it is nonetheless critical in controlling the structure of both water and ice.

In Come What May!, during the Fallarbor Contest, May's Beautifly was briefly frozen by an Ice Punch from Grace's Medicham. In A Flood of Torrential Gains!, Leon's Mr. Rime froze Ash's Gengar with Freeze-Dry during their Masters Eight Tournament battle, giving it a chance to finish off Gengar soon afterward. Mariconda S, Wang Q, Harshey RM. A mechanical role for the chemotaxis system in swarming motility. Mol Microbiol. 2006;60(6):1590–602. https://doi.org/10.1111/j.1365-2958.2006.05208.x. Shaffer M, Borton MA, McGivern BB, Zayed AA, La Rosa SL, Solden LM, et al. DRAM for distilling microbial metabolism to automate the curation of microbiome function. Nucleic Acids Res. 2020;48(16):8883–900. https://doi.org/10.1093/nar/gkaa621. Dump your ice treasures into a big tub (we use a cheap under bed storage container), add some scoops, eye droppers or small containers, and a little water to help get things melting.In Coming Full-Festival Circle!, during the Sinnoh Grand Festival semifinals, Dawn's Buneary briefly froze Jessie's Seviper with an Ice Beam. Hotaling S, Hood E, Hamilton TL. Microbial ecology of mountain glacier ecosystems: biodiversity, ecological connections and implications of a warming climate. Environ Microbiol. 2017;19(8):2935–48. https://doi.org/10.1111/1462-2920.13766. Food– Raid the freezer for things to freeze! Fish works great, you can get some from a fish market and then freeze them.